So why are we so excited about keeping LEDs at the same temperature ?
Having a stable light source is crucial in any optical measurement – whether you are measuring fluorescence, transmission, diffuse reflectance etc.
For now, lets imagine you are measuring concentration using fluorescence – maybe you are using fluorescein as marker.
Question – Is it acceptable for the light source you are using to introduce systematic errors ?
(The picture opposite is fluorescein dissolving in water, with UV illumination- pretty isn’t it ? Click the Link for a cool 5 second animation
Of course for measurements below we waited until it was uniformly distributed !)